https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:43656  17 fold change (p < 0.0001) and receiver operator characteristics area-under-curve (ROC-AUC) > 0.72. Pathway analysis of target mRNAs of these differentially expressed microRNAs showed association with the regulation of different nephrotoxic signaling pathways. In conclusion, human urinary microRNAs could identify toxic AKI early after acute injury. These urinary microRNAs have potential clinical application as early non-invasive diagnostic AKI biomarkers.]]> Tue 27 Sep 2022 14:32:55 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:10606 0.998) for paracetamol concentrations from 25 to 400 mg/L. The final yellow color was stable for at least 10 min at room temperature. There was also excellent correlation with the high-performance liquid chromatography method (r² = 0.9758). In the clinical cohort study, use of the antidote N-acetylcysteine was avoided in over a third of patients who had the plasma paracetamol concentration measured. The cost of consumables used per assay was $0.50 (US). Conclusions: This colorimetric paracetamol assay is reliable and accurate and can be performed rapidly, easily, and economically. Use of this assay in resource-poor clinical settings has the potential to have a significant clinical and economic impact on the management of paracetamol poisoning.]]> Sat 24 Mar 2018 08:13:51 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:10822 Sat 24 Mar 2018 08:13:37 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17524 2PO₄/Na₂HPO₄ and KH₂PO₄/Na₂HPO₄.2H₂O buffer solutions at pH of 6.7 and 6.9. Buffer solution containing phosphate composition of KH₂PO₄/Na₂HPO₄·2H₂O (pH=7.0) gives relatively stable values for MetHb during the storage and the amount of MetHb samples in the buffer solution retain constant up to 9 days. Therefore, stabilized MetHb blood samples can be prepared using KH₂PO₄/Na₂HPO₄·2H₂O buffer solution (pH=7) with non-ionic detergent and the samples can be stored for several days at 4–8°C.]]> Sat 24 Mar 2018 08:03:53 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34525 1.4. The diagnostic utility of WBCT20 was determined by calculating the sensitivity and specificity of the WBCT20 on admission for detecting VICC. There were 987 snake bites where both WBCT20 and PT were done on admission samples. This included 79 patients (8 %) with VICC. The WBCT20 was positive in 65/79 patients with VICC (sensitivity 82 %; 95 % confidence interval [CI]: 72-90 %) and was falsely positive in 13/908 with no coagulopathy. The WBCT20 was negative in 895/908 snake bites with no coagulopathy (specificity: 98 % 95 % CI: 97-99 %) and was falsely negative in 14/79 with VICC. Using trained clinical staff, the WBCT20 test had a relatively good sensitivity for the detection of VICC, but still missed almost one fifth of cases where antivenom was potentially indicated.]]> Fri 22 Mar 2019 13:03:57 AEDT ]]>